NifB-dependent in vitro synthesis of the iron–molybdenum cofactor ...

https://www.pnas.org/content/103/14/5297

4 Apr 2006 ... NifB-dependent in vitro synthesis of the iron–molybdenum cofactor of nitrogenase. Leonardo Curatti, Paul W. Ludden, and Luis M. Rubio.

NifB-dependent in vitro synthesis of the iron-molybdenum cofactor of ...

https://pubmed.ncbi.nlm.nih.gov/16567617/

The nitrogenase active site contains an iron and molybdenum cofactor (FeMo-co) composed of 7Fe-9S-Mo-homocitrate and one not-yet-identified ato …

NifB-Dependent “ in vitro” Synthesis of the Iron-Molybdenum ...

https://link.springer.com/chapter/10.1007/978-1-4020-8252-8_126

NifB-Dependent “in vitro” Synthesis of the Iron-Molybdenum Cofactor of Nitrogenase. Authors; Authors and affiliations. L. Curatti; P. W. Ludden; L. M. Rubio.

NifB-dependent in vitro synthesis of the iron-molybdenum cofactor of ...

https://www.researchgate.net/publication/7210928_NifB-dependent_in_vitro_synthesis_of_the_iron-molybdenum_cofactor_of_nitrogenase

3 Apr 2014 ... PDF | Biological nitrogen fixation, an essential process of the biogeochemical nitrogen cycle that supports life on Earth, is catalyzed by the.

In vitro synthesis of the iron–molybdenum cofactor of nitrogenase ...

http://www.pnas.org/content/104/45/17626.full.pdf

6 Nov 2007 ... This NifB-dependent in vitro activation of apo-NifDK required ferrous iron, sulfide, . S-adenosyl methionine (SAM), molybdate, sodium dithionite. ( ...

NifB-Dependent in vitro Synthesis of the Iron-Molybdenum Cofactor ...

https://www.jstor.org/stable/30048795

4 Apr 2006 ... NifB-dependent in vitro synthesis of the iron-molybdenum cofactor of nitrogenase . Leonardo Curatti, Paul W. Ludden, and Luis M. Rubio*.

In Vitro Synthesis of the Iron-Molybdenum Cofactor of Nitrogenase

https://www.jbc.org/content/269/2/1154.full.pdf

14 Jan 2016 ... NifB-co-dependent in vitro. FeMo-eo synthesis is absolutely dependent on the pres- ence of molybdate, homocitrate and active NIFNE pro- tein in ...

Nitrogenase cofactor biosynthesis protein NifB (IPR005980 ...

https://www.ebi.ac.uk/interpro/entry/IPR005980

NifB is a protein required for the biosynthesis of the iron-molybdenum (or ... NifB- dependent in vitro synthesis of the iron-molybdenum cofactor of nitrogenase.

Biosynthesis of the nitrogenase active-site cofactor precursor NifB ...

https://doi.org/10.1073/pnas.1904903116?utm_source=rss&utm_medium=rss

25 Nov 2019 ... Proof of in vivo NifB-co formation was additionally obtained. ... NifB-dependent in vitro synthesis of the iron-molybdenum cofactor of nitrogenase ...

In vitro synthesis of the iron-molybdenum cofactor of nitrogenase ...

https://agris.fao.org/agris-search/search.do?recordID=US9445224

In vitro synthesis of the iron-molybdenum cofactor of nitrogenase. ... NifB-co- dependent in vitro FeMo-co synthesis is absolutely dependent on the presence of  ...

Biosynthesis of the Iron-Molybdenum Cofactor of Nitrogenase ...

https://www.annualreviews.org/r/sample

13 Oct 2008 ... NifB-co-dependent in vitro FeMo-co synthesis is catalyzed by NifEN and NifH and requires the presence of molybdate, homocitrate, sodium ...

NifX and NifEN exchange NifB cofactor and the VKâ•'cluster, a newly ...

https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1365-2958.2006.05514.x

2 Nov 2006 ... The iron-molybdenum cofactor of nitrogenase. (FeMo-co) is ... NifX enhances the NifB-co-dependent in vitro synthesis of. FeMo-co. FeMo-co.

‪Leonardo Curatti‬ - ‪Google Scholar‬

http://scholar.google.com/citations?user=rwChg_wAAAAJ&hl=en

NifB-dependent in vitro synthesis of the iron–molybdenum cofactor of nitrogenase. L Curatti, PW Ludden, LM Rubio. Proceedings of the National Academy of ...

Purification and In Vitro Activity of Mitochondria Targeted ... - Frontiers

https://www.frontiersin.org/articles/10.3389/fpls.2017.01567/full

12 Sep 2017 ... NifB proteins were targeted to the mitochondria, where O2 ... The purified protein was functional in the in vitro FeMo-co synthesis assay. ... complex iron–sulfur (Fe –S) clusters called iron-molybdenum cofactor (FeMo-co) and P-cluster. ... Importantly, yNifBMi showed similar concentration-dependent activity ...

Expression and Purification of NifB Proteins from Aerobic and ...

https://experiments.springernature.com/articles/10.1007/978-1-62703-794-5_3

NifB is the key protein in the biosynthesis of nitrogenase iron-molybdenum ... LM (2006) NifB-dependent in vitro synthesis of the iron-molybdenum cofactor of ...

Substrate specificity and evolutionary implications of a NifDK ...

https://www.sciencedirect.com/science/article/pii/S0014579310001687

16 Apr 2010 ... The in vitro reconstitution of molybdenum nitrogenase was ... active site iron– molybdenum cofactor (FeMo-co) is replaced by NifB-co. ... Production of H2 by NifDK/NifB-co was stimulated by N2 and was dependent on NifH and ATP hydrolysis. ... NifB is a SAM radical enzyme that functions in the synthesis of ...

Substrate specificity and evolutionary implications of a NifDK ...

https://febs.onlinelibrary.wiley.com/doi/pdfdirect/10.1016/j.febslet.2010.02.064

26 Feb 2010 ... The in vitro reconstitution of molybdenum nitrogenase was manipulated to generate a chimeric enzyme in which ... dependent on NifH and ATP hydrolysis. Thus ... NifB is a SAM radical enzyme that functions in the synthesis of ... Abbreviations: FeMo-co, iron–molybdenum cofactor; ICP-OES, inductively cou-.

Luis M. Rubio (0000-0003-1596-2475) - ORCID | Connecting ...

https://orcid.org/0000-0003-1596-2475

NIFB-DEPENDENT "IN VITRO" SYNTHESIS OF THE IRON-MOLYBDENUM COFACTOR OF NITROGENASECurrent Plant Science and Biotechnology in ...

Structural Insights into the Mechanism of the Radical SAM Carbide ...

https://pubs.acs.org/doi/10.1021/jacs.0c02243

30 May 2020 ... (3) The latter, in addition to the Mo to V substitution, also contains a ... The NifX- like domain is not essential for NifB-co synthesis. ... The former corresponds to the cofactor responsible for SAM binding and ... of NifB variants to support NifB- dependent in vitro FeMo-co synthesis is ... M ammonium iron (III) citr.

TIGR01290

http://tigrfams.jcvi.org/cgi-bin/HmmReportPage.cgi?acc=TIGR01290

14 Feb 2011 ... Function, nitrogenase cofactor biosynthesis protein NifB ... NifB-dependent in vitro synthesis of the iron-molybdenum cofactor of nitrogenase.

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